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Send EmailWhat is LLNA (Local Lymph Node Assay)?
LLNA (Local Lymph Node Assay) is a test method used to determine skin sensitivity to chemical substances. This test is performed on mice to evaluate whether a substance causes allergic reactions on the skin. LLNA is ethically preferred because it requires less animal use than traditional skin sensitization tests.
The LLNA (Local Lymph Node Assay) calculation is a test method used to determine skin sensitivity to chemicals. Here is a general method:
Experimental Studies: In experiments conducted on mice, the substance is applied to the ears or backs of mice exposed to the chemical substance.
Lymph Node Response: After exposure, the lymph nodes of the mice are removed and cell proliferation (proliferation) is measured.
Cell Proliferation: Cell proliferation is measured by radioactive labeling or other biochemical methods.
Sensitivity Index: Using cell proliferation data, it is determined whether the chemical substance causes skin sensitivity.