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Send EmailE160a, Bromelin, Bromelains, Extranase,Traumanase, Bromelain F, bromelain Ananase, Bromelain Pineapple, Deazin, plant protein concentrate, 9001-00-7,150977-36-9
CAS: 9001-00-7;150977-36-9
Names and Identifiers
| Name | Bromelains |
| Synonyms | Deazin Bromelin Extranase 3.4.22.32 Bromelain Traumanase Bromelains Bromelain F Stem Bromelain Bromeliad enzyme bromelain Ananase Bromelain Ananase Bromelain Pineapple Plant Protein Concentrate plant protein concentrate |
| CAS | 9001-00-7 150977-36-9 |
| EINECS | 232-572-4 |
Physico-chemical Properties
| Water Solubility | soluble |
| Storage Condition | 2-8℃ |
| MDL | MFCD00081423 |
| Physical and Chemical Properties | Bromelain is a proteolytic enzyme extracted from pineapple juice or pineapple waste skin, referred to as pineapple enzyme. It is a light yellow amorphous powder. Slightly soluble in water, insoluble in ethanol, acetone, chloroform, ether. The optimum pH is 7. Slightly stinky. Enzyme activity can be activated by bovine cystine and inhibited by heavy metals. The preparation of pineapple enzyme is enteric-coated tablets, which appear grayish brown after removing the casing. Compound pineapple enzyme tablets are enteric-coated tablets of pineapple enzyme and pig bile extract powder, which show gray brown after removing the casing. |
Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R42 - May cause sensitization by inhalation |
| Safety Description | S22 - Do not breathe dust. S24 - Avoid contact with skin. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37 - Wear suitable protective clothing and gloves. |
Nature
- a proteolytic enzyme, referred to as pineapple enzyme, extracted from pineapple juice or pineapple waste skin. It is a light yellow amorphous powder with slightly abnormal odor. Slightly soluble in water, insoluble in ethanol, acetone, chloroform, ether. The optimum pH was 7 and the molecular weight was 33000.
- It is a thiol enzyme with anti-inflammatory and anti-edema effects. Enzyme activity can be activated by bovine cystine, and by heavy metal inhibition. It can hydrolyze fibrin, casein and hemoglobin, dissolve fibrin and blood clots, improve body fluid circulation, increase tissue permeability, and lead to the regression of inflammation, edema and hematoma. It is characterized by the breakdown of large molecules of fibrin, but does not destroy fibrinogen necessary for coagulation. Combined with chemotherapy drugs, can promote the penetration and diffusion of the drug to the lesion, thereby enhancing the efficacy. Unlike papain, this product can break down muscle fibers.
Use
- used for inflammation, edema, hematoma, thrombosis caused by various reasons. Bronchitis, bronchial asthma, acute Pneumonia, postpartum breast congestion, mastitis, postpartum thrombophlebitis, retinitis, etc. Combined with antibacterial drugs to treat arthritis, periarthritis, cellulitis, leg ulcers, various inflammation of the respiratory system and urinary tract infection.
- The preparation was enteric-coated tablets. The other compound pineapple enzyme tablets containing pineapple enzyme and pig bile extract powder enteric-coated tablets.
- usage and dosage of oral, 30,000~100,000 units/time, 3~4 times/day. External application of 0. 1%~ 0.2% physiological saline solution was used for wounds and chronic ulcers with more Necrotic tissues.
Safety
- can have Nausea, Vomit, Diarrhea, loss of appetite, stool thin and gastrointestinal ulcers and other gastrointestinal reactions. Occasional epistaxis, menorrhagia, dysmenorrhea and uterine bleeding. Severe liver and kidney disease or blood coagulation insufficiency should not be used.
- the patient was sealed from light and kept in a cool place.
Trait
White to light yellow powder with a slight special odor of pineapple.
Source
Pineapple.
Enzyme activity
BR，300u/mg。
soelectric point
9.55 。
Inactivation temperature
65℃。
Reference Information
| Chemical properties | bromelain is a proteolytic enzyme extracted from pineapple juice or pineapple waste skin. It is a light yellow amorphous powder. Slightly soluble in water, insoluble in ethanol, acetone, chloroform, ether. The optimum pH was 7. Slightly abnormal odor. Enzyme activity can be activated by bovine cystine, and by heavy metal inhibition. Preparation of pineapple enzyme enteric-coated tablets, remove the casing after gray-brown. The compound pineapple enzyme tablet is an enteric-coated tablet of pineapple enzyme and pig bile extract powder, which is gray-brown after removing the enteric coating. |
| Use | bromelain is commonly used in bronchitis, bronchial asthma, acute Pneumonia, postpartum breast hemorrhage, mastitis, postpartum venous thrombosis, retinitis, etc. Various causes of inflammation, edema and hematoma thrombosis. Combined with antibacterial drugs to treat arthritis, periarthritis, cellulitis, leg ulcers, various inflammation of the respiratory system and urinary tract infection. But after taking medicine, gastrointestinal reactions such as: Nausea, Ah vomiting, Diarrhea, loss of appetite, stool thin and digestive tract ulcers. Occasional epistaxis, menorrhagia, dysmenorrhea and uterine bleeding. Tablets should be swallowed, not chewable, so as not to be destroyed by pepsin and failure. Severe liver, kidney disease, is using anticoagulant or blood coagulation bacteria can not be used. The pineapple peel was washed by oral administration, and the juice was squeezed out through the Juice Extractor. 0.5g (I. E. 0.05%) of sodium benzoate was added to each liter of skin juice, which was sent to the raw material warehouse for storage at low temperature. Boropicrin sodium benzoate → press juice adsorption, elution the juice is placed in a stainless steel flask, under constant stirring, and the rotation speed is 40 ~ 60r/min, add 40g (I. E. 4%) per liter of juice the white clay, at the temperature of 100C, adsorption 15~20min, static recognition. The next day, the white clay was allowed to settle well, siphon, remove the supernatant, and leave the wet clay. Add sodium carbonate saturated solution or sodium oxyfluoride (66g/L = 6.6%) to adjust the pH value to 6.5~6~7. 50% ammonium sulfate or edible refined salt based on wet soil is further added, and the mixture is stirred for 25-30min to elute the eluate, and the eluate is pressure-filtered with a filter press to obtain a pressure-filtered solution. Juice white clay; 100 → adsorption adsorption Na2CO3;(NH4)^ 2SO4 → ^ elution; Pressure filter pressure filter solution salting out, suction filter pressure filter solution into the steel drum, adjust to pH 4.5~5 with 9% hydrochloric acid, and add 200 ~ 20% G (25% ~) Ammonium sulfate (or add 250g refined salt) to each liter of this filtrate with gentle agitation with a wooden stick. Salting out was performed to dissolve ammonium sulfate (refined salt) and put into a cold storage. The next day, the upper layer of ammonium sulfate solution was siphoned out, and the lower layer precipitated dilute enzyme paste, which was centrifuged, Crude enzyme paste was obtained. Part of the ammonium sulfate solution was removed by Suction filtration through a Buchner funnel. -20%<0 storage, to be refined. Eluent HCl;((NH4)^ 2 ^)SO4 → Salting out; Suction filtration crude product dissolution, precipitation enzyme crude product into 8~10 times the amount of tap water, then, 160g(16%) of sodium hydroxide was added to each liter of the aqueous solution of the crude enzyme to adjust the pH to 7-75, and the mixture was gently stirred until dissolved. In order to prevent foaming, rapid pressure filtration, separation and removal of impurities, to clear liquid. The pipe and filter press shall be washed with a small amount of tap water, and the washing liquid and the clarified liquid shall be combined. Under constant stirring, pH 4 was adjusted with 9% hydrochloric acid, and the enzyme was precipitated and centrifuged to obtain a wet bromelain product. Tap water of crude product; PH 7~7.5 → dissolved clear liquid HCl; PH 4 → precipitated refined product; Dry and pulverize the wet enzyme fine product; Suction filtration, which causes cracks in filter cake. The filter cake is semi-dried, and some water is removed, vacuum drying, and then grinding by ball mill, bromelain product was obtained. Calculated according to the mass of pineapple, the yield was 0.3725g/ton, and the content was 300,000 F in units/g. Oral, 3-100,000 units/time, 3-4 times/day. External application of 0.1% ~ 0.2% saline solution, for necrotic tissue more wounds and chronic ulcers. Pineapple enzyme enteric-coated tablets. Oral 1~3 tablets, 3~4 times a day, compound pineapple enzyme tablets; Oral 2~4 tablets, 3 times a day. |
| production method | Press the pineapple peel is washed, and the juice is extracted through the juice extractor, 0.5g (I. E., 0.05%) of sodium benzoate was added to each liter of skin juice as a preservative and sent to the raw material warehouse for cold storage. Boropicrin sodium benzoate → press juice adsorption, elution the juice is placed in a stainless steel flask, under constant stirring, and the rotation speed is 40 ~ 60r/min, add 40g (I. E. 4%) per liter of juice the white clay, at the temperature of 100C, adsorption 15~20min, static recognition. The next day, the white clay was allowed to settle well, siphon, remove the supernatant, and leave the wet clay. Add saturated sodium carbonate solution or sodium oxyfluoride (66g/L, 6.6%) to adjust pH 6.5~7, then add 50% ammonium sulfate or edible salt based on wet soil, stir for 25~30min, The eluent is obtained, and the eluent is pressure-filtered with a filter press to obtain a pressure-filtered liquid. Juice white clay; 100 → adsorption adsorption Na2CO3;(NH4)^ 2SO4 → ^ elution; Pressure filter pressure filter solution salting out, suction filter pressure filter solution into the steel drum, adjust to pH 4.5~5 with 9% hydrochloric acid, and add 200 ~ 20% G (25% ~) Ammonium sulfate (or add 250g refined salt) to each liter of this filtrate with gentle agitation with a wooden stick. Salting out was performed to dissolve ammonium sulfate (refined salt) and put into a cold storage. The next day, the ammonium sulfate solution in the upper layer was siphled out, and the lower layer precipitated dilute enzyme paste, which was centrifuged to obtain crude enzyme paste. Part of the ammonium sulfate solution was removed by Suction filtration through a Buchner funnel. -20%<0 storage, to be refined. Eluent HCl;(NH4) ^ 2 ^)SO4 → Salting out; The crude product was filtered to dissolve and precipitate. The crude enzyme was added to 8-10 times the amount of tap water, and then 160g(16%) was added to the aqueous solution of the crude enzyme per liter. Sodium hydroxide was adjusted to pH 7-75 and gently stirred until dissolved. In order to prevent foaming, rapid pressure filtration, separation and removal of impurities, to clear liquid. The pipe and filter press shall be washed with a small amount of tap water, and the washing liquid and the clarified liquid shall be combined. Under constant stirring, pH 4 was adjusted with 9% hydrochloric acid, and the enzyme was precipitated and centrifuged to obtain a wet bromelain product. Tap water of crude product; PH 7~7.5 → dissolved clear liquid HCl; PH 4 → precipitated refined product; Dry and pulverize the wet enzyme fine product; Suction filtration, which causes cracks in filter cake. The filter cake is semi-dried, and some water is removed, vacuum drying, and then grinding by ball mill, bromelain product was obtained. Calculated according to the mass of pineapple, the yield was 0.3725g/ton, and the content was 300,000 F in units/g. Drying of refined products; Grinding → finished products |
