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Send EmailDextranase, Endo-dextranase, 9025-95-2
Dextranase is a hydrolytic enzyme that catalyzes the breakdown of dextran, a high molecular weight polysaccharide composed of α-1,6-linked glucose units, into low molecular weight oligosaccharides. It is primarily used in the sugar industry to resolve viscosity issues caused by dextran in sugarcane juice, thereby facilitating crystallization and improving sugar quality. It is also employed in food production, biotechnology, and pharmaceutical applications to reduce viscosity and improve processing of dextran-containing solutions.
| Parameter | Information |
|---|---|
| Product Name | Dextranase |
| Other Names | Endo-dextranase, α-1,6-glucanase, Dextran endo-1,6-α-glucosidase |
| CAS Number | 9025-95-2 |
| EC Number | 3.2.1.11 |
| Enzyme Class | Hydrolase (Glycosidase) |
| Source | Microbial fermentation (typically Penicillium spp., Chaetomium spp., Aspergillus spp., Bacillus spp., Streptococcus spp.) |
| Appearance | Light yellowish to brownish powder or liquid enzyme preparation |
| Odor | Slight characteristic odor |
| Physical State (20°C) | Solid (powder) or liquid (varies by product type) |
| Type | Name |
|---|---|
| Common Names | Dextranase, Endo-dextranase, α-1,6-glucanase |
| Systematic Name | 1,6-α-D-Glucan 6-glucanohydrolase |
| EC Number | 3.2.1.11 |
| CAS Number | 9025-95-2 |
| HS Code | 3507.90 |
Dextranase is a single polypeptide chain protein with a molecular weight typically ranging from 40-100 kDa depending on the source and specific isoenzyme. It belongs to the glycoside hydrolase (GH) family, typically GH family 49, 66, or 87. The enzyme contains a catalytic domain with a characteristic (β/α)₈ barrel structure that hydrolyzes α-1,6-glycosidic bonds in dextran polymers.
Structural Features:
Dextranase
|
Catalytic Domain
|
(β/α)₈ Barrel Structure
|
Substrate Binding Site
|
α-1,6 Glycosidic Bonds
|
Internal Cleavage (Endo-Activity)
| Feature | Description |
|---|---|
| Enzyme Type | Hydrolase (Glycosidase) – endo-enzyme |
| Activity Type | Endo-α-1,6-glucanase |
| Catalytic Mechanism | Acid/base catalytic mechanism (retaining) |
| Molecular Weight | 40-100 kDa (varies by source and isoenzyme) |
| Substrate | Dextran (α-1,6-glucan polymer), isomaltooligosaccharides |
| Optimal pH | 5.0 – 6.0 |
| Optimal Temperature | 40 – 50°C |
Dextranase hydrolyzes the α-1,6-glycosidic bonds in the dextran polymer chain internally (endo-activity), releasing smaller oligosaccharides such as isomaltooligosaccharides and glucose.
General Reaction:
Dextran (α-1,6 bonds) + H₂O → Isomaltooligosaccharides + Glucose
(Substrate: Dextran T-2000, T-500, clinical dextran, etc.)
| Substrate Type | Description | Application |
|---|---|---|
| Dextran T-2000 | High molecular weight dextran (~2,000 kDa) | Sugar industry viscosity reduction |
| Dextran T-500 | Medium molecular weight dextran (~500 kDa) | Sugar processing |
| Dextran T-10 | Low molecular weight dextran (~10 kDa) | Pharmaceutical processing |
| Clinical Dextran | Dextran used in medical applications | Pharmaceutical solutions |
| Isomaltooligosaccharides | Dextran hydrolysis products | Food and prebiotic applications |
| Sugarcane Juice | Natural dextran formed in sugarcane | Sugar industry |
| Property | Value |
|---|---|
| Appearance | Light yellowish to brownish powder or liquid |
| Odor | Slight characteristic odor |
| Solubility | Soluble in water |
| Optimal pH Range | 5.0 – 6.0 |
| Effective pH Range | 4.0 – 8.0 |
| Optimal Temperature | 40 – 50°C |
| Effective Temperature Range | 20 – 60°C |
| Inactivation Temperature | >60-70°C (varies by source and formulation) |
| Isoelectric Point (pI) | 4.0 – 8.0 (varies by source) |
| Stability | Stable for 12 months when stored in a cool, dry environment |
| Parameter | Value |
|---|---|
| Activity Definition | One unit (U) is defined as the amount of enzyme that liberates 1 µmol of reducing sugar (as glucose equivalent) per minute at pH 5.5 and 40°C using dextran T-500 as substrate. |
| Typical Activity | ≥ 5,000 U/g (varies by product type) |
| Substrate | Dextran (α-1,6-glucan polymers of various molecular weights) |
| Reaction Products | Glucose, isomaltooligosaccharides (isomaltose, isomaltotriose, etc.) |
| Activators | Ca²⁺ (some enzymes), Mg²⁺ (some enzymes) |
| Inhibitors | EDTA, heavy metals (Cu²⁺, Hg²⁺, Ag⁺), substrate analogs, high dextran concentrations (substrate inhibition) |
| Step | Description |
|---|---|
| 1 | Dextranase is added to sugarcane juice or sugar solution. |
| 2 | The enzyme acts on dextran polymers (α-1,6-glucan). |
| 3 | α-1,6-Glycosidic bonds are hydrolyzed internally. |
| 4 | Dextran polymers are broken down into smaller oligosaccharides. |
| 5 | Solution viscosity is significantly reduced. |
| 6 | Filtration rate is improved. |
| 7 | Crystallization efficiency and sugar quality are enhanced. |
| 8 | Final product has improved purity and yield. |
| Application | Function |
|---|---|
| Sugarcane Juice Treatment | Breaks down dextran formed by bacterial contamination; reduces viscosity |
| Crystallization Improvement | Facilitates sugar crystallization by reducing dextran content |
| Filtration Enhancement | Improves filtration rate of sugar solutions |
| Sugar Purity Improvement | Removes dextran interference; improves final sugar quality |
| Molasses Processing | Reduces viscosity of molasses for better handling |
| Sugar Beet Processing | Dextran control in beet sugar manufacturing |
| Application | Function |
|---|---|
| Sugar Solution Purification | Increases purity of sugar solutions |
| Confectionery | Prevents dextran-induced stickiness and crystallization issues |
| Beverage Production | Reduces viscosity in dextran-containing syrups |
| Starch Processing | Removal of dextran contamination in starch-derived products |
| Application | Function |
|---|---|
| Viscosity Reduction | Reduces viscosity of dextran-containing solutions |
| Oligosaccharide Production | Production of isomaltooligosaccharides (prebiotic fibers) |
| Molecular Biology | Dextran degradation for analytical purposes |
| Polysaccharide Analysis | Structural analysis of dextran polymers |
| Application | Function |
|---|---|
| Dextran-Based Solutions | Aids in processing of dextran-containing pharmaceutical solutions |
| Blood Plasma Substitutes | Processing of clinical dextran (used in blood volume expanders) |
| Drug Delivery Systems | Dextran polymer processing for drug delivery formulations |
| Biomedical Research | Dextran degradation for research applications |
| Application | Function |
|---|---|
| Textile Processing | Dextran removal from textiles |
| Paper Industry | Dextran control in paper manufacturing |
| Wastewater Treatment | Dextran degradation in waste streams |
| Application | Recommended Dosage | Conditions |
|---|---|---|
| Sugarcane Juice Treatment | 0.001 – 0.01% of juice volume | 40-50°C, pH 5.0-6.0, 15-60 minutes |
| Sugar Solution Purification | 0.001 – 0.005% of solution volume | 40-50°C, pH 5.0-6.0 |
| Molasses Processing | 0.01 – 0.05% of molasses weight | 45-55°C, pH 5.0-6.0 |
| Pharmaceutical Processing | Varies by application | 30-40°C, pH 5.0-7.0 |
| Biotechnology Applications | 0.01 – 0.1 U/mL solution | 37-50°C, pH 5.0-6.0 |
Note: Dosage should be optimized for each specific application through trial runs.
| Parameter | Recommendation |
|---|---|
| Addition Method | Add to sugarcane juice or sugar solution during processing |
| Processing Temperature | 40-50°C (for optimum activity) |
| Reaction Time | 15-60 minutes (depends on dextran concentration) |
| pH Range | 5.0-6.0 (for optimum activity) |
| Inactivation | Inactivated at >60-70°C (during sugar evaporation or boiling) |
| Factor | Effect |
|---|---|
| Temperature | Optimal 40-50°C; activity decreases above 60°C; >70°C inactivation |
| pH | Optimal pH 5.0-6.0; significant activity at pH 4.0-8.0 |
| Substrate Concentration | Activity increases with substrate concentration; substrate inhibition at high dextran concentrations |
| Inhibitors | Heavy metals (Cu²⁺, Hg²⁺, Ag⁺), EDTA |
| Activators | Ca²⁺, Mg²⁺ (some enzymes) |
| Molecular Weight of Dextran | Higher molecular weight dextran is generally more readily hydrolyzed |
| Degree of Branching | Branched dextran may be less accessible to the enzyme |
| Parameter | Value |
|---|---|
| Acute Oral Toxicity (LD50, Rat) | > 5,000 mg/kg (very low toxicity) |
| Acute Dermal Toxicity | Low |
| Acute Inhalation Toxicity | Powder form may cause respiratory irritation |
| Skin Irritation | Mild irritant |
| Eye Irritation | May cause mild irritation |
| Skin Sensitization | May cause sensitization in sensitive individuals (protein-based) |
| Mutagenicity | Negative (Ames test) |
| Carcinogenicity | Not classified as a carcinogen |
| Reproductive Toxicity | Not classified as a reproductive toxin |
| Allergenicity | May cause allergic reactions in sensitive individuals (protein-based) |
| Hazard Class | Category | H-Statement |
|---|---|---|
| Respiratory Sensitization | Category 1 | H334 |
| Skin Sensitization | Category 1 | H317 |
| Water Hazard (WGK Germany) | 3 (severe hazard to water) | - |
Signal Word: WARNING
Hazard Pictograms: GHS07 (Exclamation Mark), GHS08 (Health)
Hazard Statements (H-Codes):
| Code | Statement |
|---|---|
| H317 | May cause an allergic skin reaction. |
| H334 | May cause allergy or asthma symptoms or breathing difficulties if inhaled. |
| Code | Statement |
|---|---|
| P261 | Avoid breathing dust/fume/gas/mist/vapors/spray. |
| P264 | Wash hands thoroughly after handling. |
| P271 | Use only outdoors or in a well-ventilated area. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P302+P352 | IF ON SKIN: Wash with plenty of soap and water. |
| P304+P340 | IF INHALED: Remove person to fresh air and keep comfortable for breathing. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present. |
| P342+P311 | If experiencing respiratory symptoms: Call a POISON CENTER/doctor. |
| P333+P313 | If skin irritation or rash occurs: Get medical advice/attention. |
| P337+P313 | If eye irritation persists: Get medical advice/attention. |
| P362+P364 | Take off contaminated clothing and wash it before reuse. |
| P501 | Dispose of contents/container in accordance with local regulations. |
| Route of Exposure | Action to Be Taken |
|---|---|
| Inhalation | Move to fresh air. If respiratory irritation persists, seek medical attention. |
| Skin Contact | Wash with plenty of soap and water. Remove contaminated clothing. If irritation persists, consult a physician. |
| Eye Contact | Rinse thoroughly with plenty of water for at least 15 minutes. Remove contact lenses. If irritation persists, seek medical attention. |
| Ingestion | Rinse mouth with water. Drink plenty of water. Do not induce vomiting. If large amount is swallowed, seek medical attention. |
| Parameter | Information |
|---|---|
| Fire Hazard | Combustible organic powder; may burn at high temperatures. |
| Hazards During Fire | Thermal decomposition produces toxic fumes including carbon monoxide, carbon dioxide, and nitrogen oxides. |
| Suitable Extinguishing Media | Water spray, CO₂, dry chemical powder, alcohol-resistant foam. |
| Special Protective Equipment | Self-contained breathing apparatus (SCBA), full protective clothing. |
| Parameter | Information |
|---|---|
| Personal Protection | Dust mask, protective goggles, gloves, coverall. |
| Ventilation | Increase ventilation; use local exhaust in confined spaces. |
| Containment | Cover spilled area to prevent dust dispersion. |
| Cleaning Methods | Collect dry using vacuum or careful sweeping. Avoid washing with water (soluble). |
| Environmental Precautions | Prevent entry into drains, sewers, and water bodies. |
| Waste Disposal | Dispose of in accordance with local regulations. |
| Parameter | Information |
|---|---|
| Storage Conditions | Store at 0-25°C in a cool, dry environment. Protect from direct sunlight. |
| Short-Term Storage | Room temperature (15-25°C) for several weeks |
| Long-Term Storage | 0-10°C recommended for extended stability (months to years) |
| Container Requirements | Keep tightly closed in original packaging. Protect from moisture and direct sunlight. |
| Materials to Avoid | Strong oxidizers, high heat, moisture, strong acids and bases |
| Shelf Life | 12 months (in unopened original packaging under proper storage) |
| Stability Note | Enzyme activity decreases over time; stable for 12 months when stored in a cool, dry environment at 0-25°C. Higher temperatures accelerate activity loss. |
| Packaging Options | Aluminum foil bags, plastic containers, drums. |
| Packaging Type | Quantity | Material |
|---|---|---|
| Aluminum Foil Bag | 1 kg, 5 kg | Laminated aluminum (moisture-proof) |
| Plastic Container | 1 kg, 5 kg, 10 kg, 25 kg | HDPE / Food-grade plastic |
| Fiber Drum | 25 kg, 50 kg | Cardboard / Plastic |
| Liquid Container | 1 L, 5 L, 10 L, 25 L | HDPE / Food-grade plastic |
| Parameter | Information |
|---|---|
| UN Number | Not applicable (not classified as dangerous goods) |
| Hazard Class | Not classified as hazardous for transport |
| Packing Group | Not applicable |
| ADR/RID | Not regulated |
| IMDG Code | Not regulated |
| IATA (Air) | Not regulated |
| Marine Pollutant | No |
| Transport Temperature | Ambient temperature (15-25°C) recommended; protect from moisture. |
| Region / Authority | Status |
|---|---|
| European Union | Approved as a food enzyme (EU 1332/2008). |
| USA (FDA) | GRAS (Generally Recognized as Safe) for food applications. |
| Turkey | Approved as a food processing aid (Turkish Food Codex). |
| EFSA | Re-evaluated and considered safe at current usage levels. |
| Kosher/Halal | Can be certified upon request (depending on source). |
| WGK Germany | 3 (severe hazard to water) |
| Parameter | Information |
|---|---|
| Aquatic Toxicity | Low toxicity to aquatic organisms. |
| Biodegradability | Readily biodegradable (protein-based). |
| Bioaccumulation | Not expected. |
| Water Hazard Class (WGK Germany) | 3 (severe hazard to water). |
| Waste Disposal | Dispose of in accordance with local regulations. Incineration is recommended for contaminated materials. |
| CAS Number | Enzyme | Description |
|---|---|---|
| 9025-95-2 | Dextranase | This CAS number is commonly used for dextranase enzyme (EC 3.2.1.11). |
Important Note: Dextranase may be obtained from various microbial sources (Penicillium, Chaetomium, Aspergillus, Bacillus, Streptococcus). The CAS number 9025-95-2 generally applies to the enzyme regardless of source. Verify the exact specification and source on the supplier's Safety Data Sheet (SDS).
| Property | Dextranase | Amylase | Cellulase | Hemicellulase |
|---|---|---|---|---|
| Enzyme Class | Hydrolase | Hydrolase | Hydrolase | Hydrolase |
| EC Number | 3.2.1.11 | 3.2.1.1 | 3.2.1.4 | Multiple |
| Substrate | Dextran (α-1,6 glucan) | Starch (α-1,4 glucan) | Cellulose (β-1,4 glucan) | Hemicellulose |
| Bond Cleaved | α-1,6 glycosidic | α-1,4 glycosidic | β-1,4 glycosidic | β-1,4 glycosidic |
| Optimal pH | 5.0-6.0 | 5.5-7.0 | 4.5-6.0 | 4.5-6.0 |
| Optimal Temperature | 40-50°C | 50-70°C | 40-60°C | 40-55°C |
| Primary Application | Sugar industry | Baking, brewing | Biofuel, juice | Baking, paper |
Q1: What is dextranase used for?
A: Dextranase is used to break down dextran, a high molecular weight polysaccharide. Its primary application is in the sugar industry to reduce viscosity caused by dextran in sugarcane juice, improve crystallization, and enhance sugar quality. It is also used in food production, biotechnology, and pharmaceutical applications.
Q2: What is the optimal pH and temperature for dextranase?
A: The optimal pH is 5.0-6.0 and the optimal temperature is 40-50°C. The enzyme is inactivated above 60-70°C.
Q3: Is dextranase safe?
A: Yes. Dextranase is considered safe for food applications (GRAS by FDA, approved by EFSA). However, the powder form may cause respiratory irritation or sensitization in sensitive individuals. Use of a mask is recommended. Avoid skin and eye contact.
Q4: How should dextranase be stored?
A: Store at 0-25°C in a cool, dry environment. Protect from direct sunlight. Keep tightly closed in original packaging. Stable for 12 months under proper storage conditions.
Q5: What is the activity of dextranase?
A: Activity varies by product type. Minimum activity is typically ≥ 5,000 U/g, but this may vary depending on the manufacturer and specific product.
Q6: What inhibits dextranase activity?
A: Heavy metals (Cu²⁺, Hg²⁺, Ag⁺), EDTA, high substrate concentrations (substrate inhibition), and strong acids or bases inhibit dextranase activity.
Q7: Can dextranase be used in other industries besides sugar?
A: Yes. Dextranase is also used in food production (sugar solution purification), biotechnology (viscosity reduction, oligosaccharide production), pharmaceutical applications (dextran-based solution processing), textile processing, paper industry, and wastewater treatment.
Q8: What is the CAS number for dextranase?
A: The CAS number for dextranase is 9025-95-2 (EC 3.2.1.11). Verify the exact CAS number on the supplier's SDS.
| Property | Value |
|---|---|
| CAS Number | 9025-95-2 |
| EC Number | 3.2.1.11 |
| Enzyme Class | Hydrolase (Glycosidase) |
| Activity Type | Endo-α-1,6-glucanase |
| Appearance | Light yellowish to brownish powder or liquid |
| Solubility | Soluble in water |
| Optimal pH | 5.0 – 6.0 |
| Effective pH Range | 4.0 – 8.0 |
| Optimal Temperature | 40 – 50°C |
| Effective Temperature Range | 20 – 60°C |
| Inactivation Temperature | >60-70°C |
| Typical Activity | ≥ 5,000 U/g |
| Substrate | Dextran (α-1,6-glucan polymers) |
| Primary Uses | Sugar industry, food production, biotechnology, pharmaceutical |
| Shelf Life | 12 months (under proper storage) |
| Storage Temperature | 0-25°C |
| UN Number | Not applicable |
| GHS Signal Word | WARNING |
| WGK Germany | 3 (severe hazard to water) |
CAS Number Verification: The CAS number for dextranase is 9025-95-2 (EC 3.2.1.11). Verify the exact CAS number and specifications on the supplier's Safety Data Sheet (SDS).
Respiratory Sensitization: Dextranase powder may cause respiratory sensitization (H334) in susceptible individuals. Use appropriate dust control measures and respiratory protection. Use of a mask is recommended.
Skin Sensitization: May cause allergic skin reactions (H317) in sensitive individuals. Use protective gloves. Avoid skin and eye contact.
Temperature Sensitivity: Dextranase is heat-sensitive. Activity decreases above 60°C and inactivation occurs above 70°C. Pay attention to processing temperatures.
Enzyme Activity: Enzyme activity depends on pH, temperature, substrate concentration, and product source. Optimize conditions for each application.
Dosage Optimization: Recommended dosages are starting points. Conduct trial runs to optimize the dosage for specific applications and products.
Substrate Specificity: Effectiveness depends on the dextran content and molecular weight distribution of the substrate. Higher molecular weight dextran is generally more readily hydrolyzed.
Water Hazard: Dextranase is classified as WGK 3 (severe hazard to water) in Germany. Prevent release into the environment.
Storage: Store at 0-25°C in a cool, dry environment. Protect from direct sunlight. Keep powder form dry. Store in tightly closed original packaging. Protect from moisture.
Handling: Use dust mask, protective gloves, and goggles. Avoid dust formation. Ensure adequate ventilation.
Sugar Industry Application: Add to sugarcane juice or sugar solution. Process at 40-50°C, pH 5.0-6.0. Allow 15-60 minutes reaction time. Inactivation occurs during sugar evaporation at >70°C.
Food Production Application: Add to sugar solutions for purification. Process at 40-50°C, pH 5.0-6.0.
Dosage: Start with recommended dosages (0.001-0.01%) and optimize through trial runs. Overdosing may cause excessive degradation and undesired effects.
Quality Control: Monitor enzyme activity and product quality regularly. Test for residual enzyme activity if required.
Waste Management: Dispose of waste in accordance with local regulations. Do not release into water bodies.
Environmental Protection: Due to WGK 3 classification, prevent entry into drains, sewers, and water bodies.
Detailed Safety: For complete safety information, consult the MSDS (Material Safety Data Sheet) provided by the manufacturer/supplier.
This Technical Data Sheet (TDS) is for informational purposes only and is prepared based on available technical data. The user is solely responsible for determining the suitability of the product for their specific application and for complying with all local, national, and international regulations. For complete safety, storage, handling, transport, waste, and regulatory compliance information, the official Safety Data Sheet (SDS/MSDS) provided by the manufacturer/supplier must be consulted. This document does not substitute professional advice. Dosage and application conditions should be optimized through product-specific trials. The CAS number for dextranase is 9025-95-2 (EC 3.2.1.11). Verify the exact specifications and source on the supplier's SDS.