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Berberine Hydrochloride, Berberine HCl, Berberine Chloride, Berberinium Chloride, 633-65-8

Berberine Hydrochloride, Berberine HCl, Berberine Chloride, Berberinium Chloride, 633-65-8

BERBERINE HYDROCHLORIDE

SECTION 1: PRODUCT IDENTITY AND CHEMICAL IDENTIFICATION

Parameter Information
Product Name Berberine Hydrochloride
Common Names Berberine chloride, Berberine HCl, Berberinium chloride
CAS Number 633-65-8
EC Number (EINECS) 211-195-9
Molecular Formula C₂₀H₁₈ClNO₄
Molecular Weight 371.81 g/mol
Chemical Class Isoquinoline alkaloid salt (quaternary ammonium salt)
Source Naturally found in BerberisCoptisHydrastis plants

SECTION 2: CHEMICAL STRUCTURE

                    OCH₃
                      |
    O—CH₂—O—[A]——[B]—C
               \     /
                C    C
               /      \
          [C]          [D]
             \        /
              C——N⁺——C
             /        \
        O—CH₂—O—[E]  [F]—OCH₃
                      |
                     OCH₃

    Berberine Chloride
    C₂₀H₁₈ClNO₄
    
    Quaternary isoquinoline alkaloid
    (Protoberberine skeleton)

Simplified Chemical Structure:

                  OCH₃
                   |
    OCH₃—[D]——[C]——[B]——[A]—O—CH₂—O
                |     |
                N⁺    |
               /      |
    OCH₃—[F]——[E]——[A]—O—CH₂—O
                    |
                   OCH₃

    Berberine Ion (Anion: Cl⁻)
Parameter Description
Molecular Formula C₂₀H₁₈ClNO₄
Molecular Weight 371.81 g/mol
Chemical Class Isoquinoline alkaloid (quaternary ammonium)
Skeleton Protoberberine derivative
SMILES COC1=CC=2C(=C1)C3=CC4=C(C=C3CCN2C)OCO4.Cl
InChI InChI=1S/C20H18NO4.ClH/c1-22-15-4-3-12-7-14-10-17-16(24-11-25-17)8-13(14)5-6-21(12)9-15;/h3-4,8-10H,5-7,11H2,1-2H3;1H/q+1;/p-1
InChI Key ZDFRQCUZMYEECK-UHFFFAOYSA-M
Hydrogen Bond Donors 0
Hydrogen Bond Acceptors 5
Rotatable Bond Count 3
Polar Surface Area (PSA) 40.8 Ų
Charge Quaternary ammonium (N⁺) + chloride (Cl⁻)

SECTION 3: PHYSICAL AND CHEMICAL PROPERTIES

Property Value Test Method / Note
Appearance Yellow-orange crystalline powder Visual
Odor Slight characteristic odor Olfactometric
Molecular Weight 371.81 g/mol Calculated
Melting Point 250-260°C (decomposes) DSC / Capillary
Density (20°C) ~1.5 g/cm³ (estimated) -
Solubility in Water (20°C) Soluble (~10-15 g/L) General Method
Solubility in Hot Water More soluble General Method
Solubility in Ethanol Slightly soluble General Method
Solubility in Methanol Soluble General Method
Solubility in DMSO Soluble General Method
Solubility in Chloroform Very slightly soluble General Method
Log P (Octanol/Water) ~1.0 (quaternary charge; hydrophilic) Calculated
pH (Aqueous, 1% solution) ~4.0-5.5 (acidic) pH Meter
Flash Point >150°C -
Vapor Pressure (25°C) Very low -
Thermal Stability Stable up to 200°C; decomposes at higher temperatures -
Hygroscopicity Slightly hygroscopic -
Optical Activity Not optically active -
Crystal System Monoclinic XRD

SECTION 4: SPECTROSCOPIC PROPERTIES

Parameter Value Description
UV-VIS Absorption (λmax) ~230 nm, ~265 nm, ~348 nm, ~420 nm In ethanol or methanol
Molar Absorptivity (ε) Variable (concentration dependent) -
IR (KBr, cm⁻¹) ~3400, 2940, 1720, 1620, 1510, 1460, 1360, 1250, 1200, 1100, 1040, 940 Characteristic alkaloid peaks
NMR (¹H, DMSO-d6) δ 9.9 (s, 1H), 8.7 (s, 1H), 7.8 (s, 1H), 7.5 (m), 6.9 (s), 6.3 (s), 4.9 (s, 2H), 3.8-4.0 (m, 9H) -
Fluorescence Strong yellow-green fluorescence (excitation at 360 nm) In aqueous solution
LC-MS (ESI+) [M⁺] m/z 336 (berberine ion) Chloride ion dissociates

SECTION 5: PHARMACOLOGICAL PROPERTIES AND MECHANISM OF ACTION

Property Description
Antimicrobial Activity Effective against Gram-positive bacteria (S. aureusS. pneumoniaeC. acnes) and some Gram-negative bacteria. Acts through DNA intercalation and cell membrane permeability.
AMPK Activation Activates the AMPK (AMP-activated protein kinase) pathway, regulating cellular energy homeostasis; increases mitochondrial oxidation.
Glucose Metabolism Increases insulin sensitivity; stimulates glucose uptake; inhibits hepatic gluconeogenesis.
Lipid Metabolism Reduces LDL-cholesterol and triglyceride levels; reduces PCSK9 expression; reduces hepatic steatosis.
Anti-inflammatory Effect Inhibits NF-κB and MAPK pathways; reduces pro-inflammatory cytokines (TNF-α, IL-6, IL-1β).
Antioxidant Activity Scavenges reactive oxygen species (ROS); increases endogenous antioxidant enzymes (SOD, GSH-Px, CAT).
Neuroprotective Effect Reduces neuroinflammation; inhibits β-amyloid aggregation in Alzheimer's disease models.
Cardiovascular Effects Reduces blood pressure; improves endothelial function; induces vasodilation.
DNA Intercalation Intercalates into DNA via quaternary ammonium structure; inhibits topoisomerase I and II.

SECTION 6: COMMERCIAL GRADES AND VARIANTS

Grade / Type Purity Appearance Primary Application
Pharmaceutical Grade (USP/EP) ≥98.0% Yellow-orange crystalline powder Supplements, pharmaceutical studies
Food Supplement Grade ≥97.0% Yellow-orange powder Dietary supplements, capsules
Research/Analytical Grade ≥99.0% (HPLC) Yellow-orange crystals Analytical standards, R&D
Industrial Grade ≥95.0% Yellowish-orange powder Plant extract production, technical applications
Micronized Grade ≥98.0% Micronized powder Advanced formulations (lipid nanoparticles)

SECTION 7: CLINICAL RESEARCH AND INDICATIONS

Indication / Use Mechanism of Action Research Status Typical Dose
Metabolic Syndrome / Type 2 Diabetes AMPK activation, glucose metabolism, insulin sensitivity Clinical trials (Phase II-III) 500-1500 mg/day
Hyperlipidemia PCSK9 reduction, LDL and triglyceride lowering Clinical trials 500-1000 mg/day
Hypertension Vasodilation, ACE inhibition (limited) Preclinical / Clinical pilot 500-1000 mg/day
Gastrointestinal Infections Antimicrobial (bacterial diarrhea, C. difficile) Preclinical / Clinical 200-500 mg/day
Acne / Dermatological Antimicrobial, anti-inflammatory (C. acnes) Preclinical / Topical Variable
Neurodegenerative Diseases Neuroprotective, β-amyloid inhibition Preclinical -
Polycystic Ovary Syndrome (PCOS) Metabolic regulation, insulin sensitivity Clinical pilot 500-1500 mg/day
Non-alcoholic Fatty Liver Disease (NAFLD) Lipid metabolism, AMPK activation Clinical trials 500-1000 mg/day

SECTION 8: ORAL BIOAVAILABILITY AND FORMULATION NOTES

Parameter Information
Oral Bioavailability Low (~1-5%); P-glycoprotein efflux; hepatic first-pass metabolism (CYP2C9, CYP3A4, CYP2D6)
Absorption Small intestine (duodenum, jejunum)
Plasma Peak Concentration (Cmax) 1-3 hours (oral administration)
Plasma Half-Life (t½) ~2-5 hours (human)
Elimination Hepatic metabolism; renal excretion (urine); feces
Volume of Distribution (Vd) Extensive (tissue distribution)
Protein Binding ~50-60%
Bioavailability Enhancement Strategies • Combination with piperine (P-gp inhibition)
• Nanocarrier systems (lipid nanoparticles, micelles)
• Phytosome technology
• Amorphous solid dispersion
• Solubility enhancers in small intestine
Formulation Types • Capsules (powder, granules)
• Tablets (direct compression)
• Lipid-based formulations
• Nanoparticulate systems
• Topical gels and creams

SECTION 9: TYPICAL DOSAGE AND USAGE

Route of Administration Typical Dose Range Frequency Notes
Oral (Metabolic Syndrome) 500-1500 mg 2-3 times daily Take with meals
Oral (Lipid Lowering) 500-1000 mg 2 times daily 6-12 weeks; physician supervision
Oral (Antimicrobial) 200-500 mg 2-4 times daily Variable; acute infection
Oral (Maintenance / Support) 300-600 mg Once daily Long-term use
Topical (Acne) 1-5% 1-2 times daily Gel, cream formulations
Topical (Wound) 0.5-2% 2 times daily Antimicrobial

SECTION 10: SAFETY PROFILE AND TOXICOLOGICAL ASSESSMENT

Parameter Value / Description
GHS Classification Warning (irritant)
Signal Word Warning
Hazard Pictograms GHS07 (Exclamation mark)
Hazard Statements (H-Codes) H315 (Causes skin irritation), H319 (Causes serious eye irritation), H335 (May cause respiratory irritation)
Precautionary Statements (P-Codes) P261, P264, P280, P302+P352, P305+P351+P338, P312
Acute Oral Toxicity (LD50, Rat) ~500-1000 mg/kg (moderate toxicity)
Acute Dermal Toxicity (LD50, Rat) >2000 mg/kg (low toxicity)
Acute Inhalation Toxicity Low toxicity
Skin Irritation Mild irritant
Eye Irritation Moderate irritant
Skin Sensitization Low potential
Carcinogenicity Not classified as carcinogenic
Mutagenicity In vitro mutagenic potential (AMES test positive); in vivo studies inconclusive
Reproductive Toxicity Embryotoxic potential at high doses (animal)
Target Organs Liver, kidneys, gastrointestinal system
NOAEL (Rat, 90 days) ~100-300 mg/kg/day
Topical Safety Well tolerated; concentration dependent
Pregnancy Category Category C (USA) - risk in animal studies
Lactation Insufficient data; should not be used
Drug Interactions CYP3A4, CYP2D6, CYP2C9 inhibitor; P-gp substrate/inhibitor
Common Side Effects GI distress, diarrhea, constipation, nausea (dose dependent)
Serious Side Effects Hepatotoxicity at high doses, QT prolongation (rare)

SECTION 11: DRUG INTERACTIONS

Drug / Substance Interaction Type Severity Management
Antidiabetics (Metformin, Sulfonylureas) Increased hypoglycemia risk Moderate Blood glucose monitoring; dose adjustment
Anticoagulants (Warfarin) Increased bleeding risk Moderate INR monitoring
Antihypertensives (Calcium channel blockers) Hypotension risk Low Blood pressure monitoring
CYP3A4 Substrates (Statins, CCB, Cyclosporine) Increased plasma levels Moderate Dose adjustment; monitoring
CYP2D6 Substrates (Beta-blockers, Antidepressants) Increased plasma levels Low-Moderate Monitoring; dose adjustment
P-gp Substrates (Digoxin, Talinolol) Increased absorption, decreased clearance Moderate Monitoring; dose adjustment
Piperine (Black Pepper Extract) Increased berberine bioavailability Positive Combination products
Erythromycin / Clarithromycin Hepatotoxicity risk Moderate Avoid co-administration
Alcohol Hepatotoxicity risk Moderate Avoid alcohol consumption

SECTION 12: CLINICAL STUDY SUMMARY

Study Topic Result Evidence Level Reference
Type 2 Diabetes (Berberine) HbA1c ↓ 1-2%; FPG ↓ ~30% Randomized Controlled Trial (RCT) J Clin Endocrinol Metab. 2012
Hyperlipidemia LDL ↓ ~20%; TG ↓ ~30% RCT Am J Cardiol. 2009
NAFLD (Fatty Liver) Liver steatosis ↓; ALT ↓ RCT J Hepatol. 2016
PCOS (Polycystic Ovary) Insulin sensitivity ↑; LH/FSH ↓ RCT Exp Clin Endocrinol Diabetes. 2012
Hypertension Systolic BP ↓ ~10 mmHg; Diastolic BP ↓ ~5 mmHg Meta-analysis Phytother Res. 2015
Metabolic Syndrome Waist circumference ↓; TG ↓; HDL ↑ RCT J Diabetes. 2017
Acne (Topical) Inflammatory lesions ↓ ~50-70% Preclinical / Pilot J Dermatolog Treat. 2020
Neuroprotection (Alzheimer's) β-amyloid aggregation ↓ Preclinical J Neuroinflammation. 2019

SECTION 13: FIRST AID MEASURES

Route of Exposure Action
Inhalation If inhaled as powder, move to fresh air. Seek medical attention if respiratory irritation or symptoms persist.
Skin Contact Wash with plenty of soap and water. Remove contaminated clothing. Seek medical attention if irritation develops.
Eye Contact Rinse thoroughly with plenty of water for at least 15 minutes. Keep eyelids open. Remove contact lenses if present. Seek medical attention if irritation persists.
Ingestion Rinse mouth with water. Do NOT induce vomiting. Drink 1-2 glasses of water. Seek immediate medical attention.
Note Symptomatic treatment is administered. No specific antidote available. Gastrointestinal decontamination may be required (large dose).

SECTION 14: ADVERSE EFFECTS AND MANAGEMENT

Adverse Effect Frequency Severity Management
Gastrointestinal Discomfort (stomach pain) Common (>10%) Mild-Moderate Take with meals; reduce dose
Diarrhea Common (5-15%) Mild-Moderate Increase fluid intake; probiotics
Constipation Common (5-10%) Mild Increase fiber intake
Nausea Common (5-10%) Mild Take with meals; divide dose
Dizziness Less common (2-5%) Mild Rest; hypotension monitoring
Elevated Liver Transaminases Less common Moderate Liver function tests; high-dose monitoring
Skin Rash Rare (<1%) Mild Antihistamine; may require discontinuation
QT Prolongation Very rare (<0.1%) Serious ECG monitoring; avoid high doses

SECTION 15: STORAGE AND SHELF LIFE

Parameter Information
Storage Conditions Store in a cool (<25°C), dry, dark place; protect from direct sunlight, heat, and moisture.
Temperature Limits 15-25°C (ideal); do not exceed 30°C
Light Protect from UV light and direct sunlight (photodegradation)
Humidity Low humidity environment (slightly hygroscopic)
Oxygen Protect against oxidation; keep containers tightly closed
Incompatible Materials Strong oxidizing agents, strong alkalis
Container Requirements Light-proof, moisture-barrier containers: Amber glass jars, aluminum bags, opaque HDPE
Shelf Life 24-36 months (unopened original packaging, under proper storage conditions)
Shelf Life (After Opening) 12-24 months (under proper storage conditions)
Signs of Degradation Darkening color (dark orange-brown); caking; decreased solubility; HPLC impurity increase
Stability Warning May degrade under light and humidity; oxidized product may form colored impurities

SECTION 16: PACKAGING OPTIONS

Packaging Type Quantity / Capacity Material Application Area
Amber Glass Jar 50 g, 100 g, 500 g, 1 kg Amber glass (UV protected) Pharmaceutical, laboratory, high purity
Aluminum Foil Bag 1 kg, 5 kg, 10 kg, 25 kg Aluminum / PE (light and moisture proof) Supplement production, industrial
Plastic Drum (HDPE) 25 kg, 50 kg HDPE (opaque, moisture barrier) Industrial bulk supply
Kraft Bag (PE Lined) 10 kg, 25 kg Kraft paper / PE liner Economical packaging
Fiber Drum 25 kg, 50 kg Fiber / PE liner Industrial supply
Amber Glass Vial 10 g, 25 g, 50 g Amber glass (UV protected) Analytical standards, R&D

SECTION 17: TRANSPORTATION INFORMATION

Parameter Information
UN Number Not applicable (not classified as dangerous substance)
Hazard Class Not applicable
Packing Group Not applicable
ADR/RID Not classified as dangerous goods
IMDG Code Not marine pollutant
IATA (Air) Not classified as dangerous goods; can be transported by air
Transport Temperature Ambient temperature; protect from heat, moisture, and light
Transport Precautions Keep away from strong oxidizing agents; light-proof packaging
Spill Cleanup Collect mechanically; clean with absorbent material; avoid dust generation
Marine Pollutant No

SECTION 18: QUALITY CONTROL AND ANALYTICAL METHODS

Parameter Test Method Specification
Appearance Visual Yellow-orange crystalline powder
Assay (Berberine HCl) HPLC / UV ≥98.0% (Pharm.); ≥97.0% (Supplement)
Berberine Free Base HPLC ≤0.5%
Melting Point DSC / Capillary 250-260°C (decomposes)
Water Content Karl Fischer ≤1.0% (Pharm.); ≤2.0% (Supplement)
Residue on Ignition Gravimetric ≤0.5% (Pharm.); ≤1.0% (Supplement)
Heavy Metals (Pb) ICP-MS / USP <231> ≤10 ppm
Arsenic (As) ICP-MS ≤2 ppm
Chloride Content Titration 9.0-10.0%
HPLC Impurities HPLC Single impurity ≤0.5%; Total ≤2.0%
UV-VIS Spectrum UV-VIS λmax 348 ± 2 nm (methanol)
Particle Size Laser Diffraction As per customer specification
Microbiological Purity USP <61> TAMC ≤10² CFU/g; TYMC ≤10¹ CFU/g
E. coli USP <62> Negative
Salmonella USP <62> Negative

SECTION 19: QUALITY SPECIFICATIONS

Parameter Specification (Pharmaceutical) Specification (Supplement) Test Method
Appearance Yellow-orange crystalline powder Yellow-orange powder Visual
Assay (HPLC) ≥98.0% ≥97.0% HPLC
Berberine Free Base ≤0.5% ≤1.0% HPLC
Water Content ≤1.0% ≤2.0% Karl Fischer
Residue on Ignition ≤0.5% ≤1.0% Gravimetric
Heavy Metals (Pb) ≤10 ppm ≤20 ppm ICP-MS
Arsenic (As) ≤2 ppm ≤5 ppm ICP-MS
Chloride Content 9.0-10.0% 8.5-10.5% Titration
Single Impurity ≤0.5% ≤1.0% HPLC
Total Impurities ≤2.0% ≤3.0% HPLC
Microbiological Purity TAMC ≤10² CFU/g TAMC ≤10³ CFU/g USP <61>

SECTION 20: OTHER NAMES AND SYNONYMS

Type Name
Chemical Name Berberine Chloride
Common Names Berberine HCl, Berberine Chloride, Berberinium chloride
IUPAC Name 5,6-Dihydro-9,10-dimethoxybenzo[g]-1,3-benzodioxolo[5,6-a]quinolizinium chloride
Trade Marks Berberine HCl, Berberine Hydrochloride (bulk), Berberine Complex
Plant Sources Berberis aristata (Indian barberry), Coptis chinensis (Goldthread), Hydrastis canadensis (Goldenseal)
CAS Number 633-65-8
EC Number 211-195-9
PubChem CID 2353
DrugBank ID DB04115
UNII (FDA) 96L0B2HBIZ

SECTION 21: GHS CLASSIFICATION AND LABELING

Parameter Information
GHS Classification Skin Irritant Category 2; Eye Irritant Category 2A; STOT SE3
Signal Word Warning
Hazard Pictograms GHS07 (Exclamation mark)
Hazard Statements (H-Codes) H315 (Causes skin irritation), H319 (Causes serious eye irritation), H335 (May cause respiratory irritation)
Precautionary Statements (P-Codes) P261, P264, P271, P280, P302+P352, P305+P351+P338, P312, P332+P313, P337+P313, P362+P364, P403+P233, P405, P501
NFPA 704 Classification Health: 1, Flammability: 1, Reactivity: 0

SECTION 22: ENVIRONMENTAL IMPACT AND DISPOSAL

Parameter Information
Biodegradability Partially biodegradable (alkaloid natural compound)
Aquatic Toxicity (EC50) >10 mg/L (Daphnia magna) - moderate toxicity
Aquatic Toxicity (LC50) >10 mg/L (fish) - moderate toxicity
Soil Mobility Low (quaternary ammonium charge)
Bioaccumulation Potential Low-Moderate
Disposal Method Dispose of in accordance with local, regional, and national regulations
Incineration Controlled incineration facilities; energy recovery possible
Sewer Discharge Do not discharge into sewers or water resources
Environmental Precautions Prevent environmental spread during spills

SECTION 23: FREQUENTLY ASKED QUESTIONS

Question Answer
Q1: What is Berberine HCl? Berberine HCl is the hydrochloride salt of an isoquinoline alkaloid naturally found in many plants. It is known for its antimicrobial, metabolic regulatory, and anti-inflammatory properties.
Q2: What is Berberine HCl used for? It is being researched for metabolic syndrome, type 2 diabetes, hyperlipidemia, hypertension, gastrointestinal infections, and PCOS.
Q3: Is Berberine HCl safe? At recommended doses (500-1500 mg/day), it is generally safe. GI discomfort is the most common side effect. Hepatotoxicity and QT prolongation are risks at high doses.
Q4: Is Berberine HCl bioavailability low? Yes, oral bioavailability is very low (~1-5%). Combination with piperine (black pepper extract) and nanoparticulate systems improve bioavailability.
Q5: Is Berberine HCl good for diabetes? Clinical studies show that berberine lowers blood sugar (HbA1c) and increases insulin sensitivity. It may have comparable efficacy to metformin.
Q6: Does Berberine HCl interact with other medications? Yes, it is a CYP3A4, CYP2D6, CYP2C9 inhibitor and P-gp substrate/inhibitor. It may interact with antidiabetics, anticoagulants, statins, and digoxin.
Q7: Does Berberine HCl help with weight loss? Studies show it may help with modest weight loss through AMPK activation and lipid metabolism regulation.
Q8: Is Berberine HCl safe during pregnancy? FDA Pregnancy Category C. Embryotoxicity has been reported in animal studies. It should not be used during pregnancy and lactation.
Q9: Can Berberine HCl be used on the skin? Yes, it is being researched for acne treatment (1-5% topical formulations) due to its antimicrobial and anti-inflammatory effects.
Q10: Is Berberine HCl a natural compound? Yes, it naturally occurs in plants such as BerberisCoptis, and Hydrastis. Commercial berberine is typically extracted from these plants.

SECTION 24: QUICK REFERENCE TABLE

Property Value
CAS Number 633-65-8
Molecular Formula C₂₀H₁₈ClNO₄
Molecular Weight 371.81 g/mol
Appearance Yellow-orange crystalline powder
Melting Point 250-260°C (decomposes)
Water Solubility Soluble (~10-15 g/L)
Log P ~1.0 (quaternary charge)
Primary Function Antimicrobial, metabolic regulator
Typical Oral Dose 500-1500 mg/day
Shelf Life 24-36 months (proper storage)
Storage Cool (<25°C), dry, dark, light-proof
GHS Classification Irritant (GHS07)
FDA Approval Not an FDA-approved drug; sold as dietary supplement
Bioavailability Low (~1-5%)
Main Applications Metabolic support, antimicrobial, cardiovascular support

SECTION 25: CRITICAL WARNINGS AND BEST PRACTICES

CRITICAL WARNINGS:

  1. Low bioavailability: Oral berberine bioavailability is very low. Bioavailability enhancement strategies (piperine, nanoparticles, amorphous dispersion) are critical in formulations.

  2. Drug interactions: Has potential for interaction with CYP3A4, CYP2D6, CYP2C9, CYP1A2, and P-gp pathways. Caution should be exercised in patients taking concomitant medications.

  3. Liver toxicity: High doses may elevate liver transaminases. Should be used with caution in patients with liver disease.

  4. QT prolongation: QT interval prolongation has been reported at high doses. ECG monitoring is recommended in patients with cardiac conditions.

  5. Gastrointestinal side effects: Nausea, diarrhea, and abdominal pain are common. Taking with meals and starting with a low dose is recommended.

  6. Pregnancy and lactation: FDA Pregnancy Category C. Should not be used during pregnancy and lactation.

  7. OTC status: Berberine is not a drug, but a dietary supplement. It cannot be sold with therapeutic claims.

  8. Regulatory compliance: In Turkey, it can be sold as a dietary supplement; however, permission from the Turkish Ministry of Health is required for pharmaceutical use.

BEST PRACTICE RECOMMENDATIONS:

  1. Bioavailability-enhancing formulations: Combination with piperine (5-10%); nanocarrier systems (lipid nanoparticles, micelles); amorphous solid dispersions; phytosome technology.

  2. Gradual dose increase: Start at low dose (200-300 mg/day); increase to 1500 mg/day over 4-6 weeks as tolerated.

  3. Take with meals: Take with food to improve gastrointestinal tolerance.

  4. Divide daily dose: Divide the dose 2-3 times daily (e.g., 500 mg x 3 times).

  5. Quality control: HPLC assay of berberine; loss on drying; microbiological purity; heavy metals; PAH content.

  6. Stability studies: Degradation profile under light, humidity, and temperature; shelf life determination.

  7. Safety evaluation: Hepatic and renal function tests; QT monitoring (high dose); drug interaction studies.

  8. Contraindications: Pregnancy, lactation, severe liver/kidney disease, history of QT prolongation, patients using CYP inhibitors and P-gp substrates.

  9. Clinical study compliance: Refer to clinical doses on product labels; avoid therapeutic claims.

  10. Microbiological control: Berberine HCl powder should be controlled for microbiological purity (TAMC, TYMC, pathogens).

LEGAL DISCLAIMER:

The information provided in this document is based on our current knowledge and experience and is presented in good faith; however, it does not constitute a binding specification or guarantee as all conditions of use are beyond our control. This Technical Data Sheet is for informational purposes only. Users are responsible for testing suitability for their own applications. For complete safety, storage, use, handling, waste, and regulatory compliance information, please refer to the official Safety Data Sheet (SDS/MSDS) provided by the manufacturer/supplier. This product is a dietary supplement and should not be used with therapeutic claims.

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