Methylenebisacrylamide, N,N'-Methylene Diacrylamide, Bisacrylamide, Methylenebisacrylamide, Bis-acrylamide, MBAA, 110-26-9
N,N'-METHYLENEBISACRYLAMIDE (MBA)
CAS Number: 110-26-9
EC Number (EINECS): 203-750-9
Molecular Formula: C₇H₁₀N₂O₂
Molecular Weight: 154.17 g/mol
Chemical Class: Diacrylamide (crosslinking monomer)
SECTION 1: PRODUCT IDENTIFICATION AND CHEMICAL IDENTITY
| Parameter |
Description |
| Chemical Name (IUPAC) |
N-[(Prop-2-enoylamino)methyl]prop-2-enamide |
| Other Names |
MBA, N,N'-Methylenebisacrylamide, N,N'-Methylene Diacrylamide, Bisacrylamide, Methylenebisacrylamide, MBAA, Bis-acrylamide |
| CAS Number |
110-26-9 |
| EC Number (EINECS) |
203-750-9 |
| Molecular Formula |
C₇H₁₀N₂O₂ |
| Molecular Weight |
154.17 g/mol |
| Chemical Class |
Diacrylamide (crosslinking monomer) |
| Structure |
CH₂=CH-C(=O)-NH-CH₂-NH-C(=O)-CH=CH₂ |
| Appearance |
White crystalline particles |
| Physical State |
Solid |
| Odor |
Neutral |
| Purity |
≥ 99.0% |
SECTION 2: PHYSICAL AND CHEMICAL PROPERTIES
| Property |
Value / Range |
| Molecular Formula |
C₇H₁₀N₂O₂ |
| Molecular Weight |
154.17 g/mol |
| Appearance |
White crystalline particles |
| Physical State |
Solid |
| Odor |
Neutral |
| Density (30°C) |
~1.235 g/cm³ |
| Melting / Freezing Point |
~185°C |
| Purity |
≥ 99.0% |
| Solubility in Water |
Soluble (~30 g/L, 20°C) |
| Solubility in Organic Solvents |
Soluble in methanol, DMF, DMSO; slightly soluble in acetone |
| Thermal Decomposition |
> 200°C releases nitrogen oxides (NOₓ) and CO₂ |
SECTION 3: SOLUBILITY PROFILE
| Solvent |
Solubility |
Condition |
| Water |
Soluble (~30 g/L) |
20°C |
| Methanol |
Soluble |
- |
| DMF |
Soluble |
- |
| DMSO |
Soluble |
- |
| Acetone |
Slightly soluble |
- |
| Ethanol |
Slightly soluble |
- |
Critical Note: MBA is soluble in water and most polar organic solvents. This property makes it an ideal crosslinker for aqueous polymerization systems and biochemical applications.
SECTION 4: CHEMICAL PROPERTIES AND REACTIONS
| Property |
Description |
| Chemical Class |
Diacrylamide (crosslinking monomer) |
| Functional Groups |
Two acrylamide groups (C=C), amide bonds |
| Reactivity |
High; readily polymerizes |
| Polymerization |
Free-radical polymerization (solution, gel, emulsion) |
| Crosslinking |
Forms crosslinks between polymer chains |
| Copolymerization |
Copolymerizes with acrylamide, acrylates, and other vinyl monomers |
| Thermal Decomposition |
> 200°C releases nitrogen oxides (NOₓ) and CO₂ |
| Hydrolysis |
Slow hydrolysis in acidic/basic media |
| Oxidation |
Stable under normal conditions |
4.1. Key Reactions
| Reaction Type |
Description |
| Radical Polymerization |
Forms crosslinked polymer networks |
| Copolymerization |
With acrylamide, acrylates, methacrylates |
| Hydrolysis |
Hydrolyzes to acrylic acid derivatives in acidic/basic media |
| Crosslinking |
Forms permanent crosslinks between polymer chains |
SECTION 5: FUNCTIONAL PROPERTIES
| Property |
Description |
| Crosslinker |
Effective crosslinking agent in polymer systems |
| High Reactivity |
High polymerization reactivity |
| Gel Formation |
Crosslinker in polyacrylamide gels |
| Mechanical Strength |
Increases mechanical strength of crosslinked polymers |
| Chemical Resistance |
Increases chemical resistance of crosslinked networks |
SECTION 6: MAJOR APPLICATIONS AND END-USES
6.1. Polyacrylamide Gel Electrophoresis (Most Important Application)
| Application |
Function |
Suitability |
| SDS-PAGE |
Gel crosslinker for protein analysis |
High |
| Native Gel Electrophoresis |
Gel crosslinker for protein analysis |
High |
| DNA Electrophoresis |
Gel crosslinker for DNA analysis |
High |
| 2D Electrophoresis |
Gel crosslinker for protein analysis |
High |
6.2. Biochemistry and Molecular Biology
| Application |
Function |
Suitability |
| Protein Purification |
Affinity chromatography gels |
High |
| Enzyme Studies |
Gel immobilization |
High |
| DNA Analysis |
DNA gel electrophoresis |
High |
6.3. Polymer and Resin Production
| Application |
Function |
Suitability |
| Polyacrylamide Gels |
Crosslinker |
High |
| Superabsorbent Polymers |
Crosslinker |
High |
| Ion Exchange Resins |
Crosslinker |
High |
| Controlled Release Systems |
Crosslinker |
High |
6.4. Photography and Imaging
| Application |
Function |
Suitability |
| Photographic Nylon |
Photographic plastic raw material |
High |
| Photographic Plastics |
Imaging materials |
High |
| Plate Making |
Plate production |
High |
6.5. Water Treatment and Wastewater
| Application |
Function |
Suitability |
| Wastewater Treatment |
Flocculant gels |
High |
| Coagulation |
Polymer aids |
High |
6.6. Casting and Coating
| Application |
Function |
Suitability |
| Casting |
Mold materials |
High |
| Coating |
Protective coatings |
High |
6.7. Printing and Graphic Arts
| Application |
Function |
Suitability |
| Printing |
Printing plates |
High |
| Graphic Arts |
Imaging materials |
High |
SECTION 7: INDUSTRY SUITABILITY MATRIX
| Industry |
Suitability |
Explanation |
| Biochemistry / Molecular Biology |
High |
Gel electrophoresis, protein purification |
| Polymer Chemistry |
High |
Crosslinker, gel production |
| Water Treatment |
High |
Flocculant gels |
| Photography |
High |
Photographic nylon, plate making |
| Printing |
High |
Printing plates |
| Coatings |
High |
Protective coatings |
| Pharmaceuticals |
High |
Controlled release systems |
| Cosmetics |
Not suitable |
Not used in cosmetics |
| Food |
Not suitable |
Not used as food additive |
SECTION 8: OTHER NAMES AND IDENTIFIERS
| Type |
Name / Description |
| IUPAC Name |
N-[(Prop-2-enoylamino)methyl]prop-2-enamide |
| Commercial Names |
MBA, MBAA, Bisacrylamide |
| Synonyms |
N,N'-Methylenebisacrylamide, N,N'-Methylene Diacrylamide, N,N'-Methylenebis(acrylamide), Methylenebisacrylamide, Bis-acrylamide |
| CAS Number |
110-26-9 |
| EC Number |
203-750-9 |
| HS Code |
29241900 |
SECTION 9: TOXICOLOGY AND HEALTH EFFECTS
| Parameter |
Value / Description |
| GHS Classification |
Warning |
| Hazard Statements |
H302 (Harmful if swallowed), H315 (Causes skin irritation), H319 (Causes serious eye irritation), H335 (May cause respiratory irritation), H341 (Suspected of causing genetic defects) |
| Precautionary Statements |
P201 (Obtain special instructions before use), P261 (Avoid breathing dust), P280 (Wear protective gloves/protective clothing/eye protection/face protection), P302+P352 (IF ON SKIN: Wash with plenty of water), P305+P351+P338 (IF IN EYES: Rinse cautiously with water) |
| Oral LD50 (Rat) |
~400-500 mg/kg |
| Dermal LD50 (Rabbit) |
> 2,000 mg/kg |
| Skin Irritation |
Irritant |
| Eye Irritation |
Irritant |
| Skin Sensitization |
Positive (allergic contact dermatitis) |
| Mutagenicity |
Suspected (H341) |
| Carcinogenicity |
Not classified |
| Target Organs |
Skin, eyes, respiratory system |
SECTION 10: SAFETY AND STORAGE
| Parameter |
Information |
| Storage Conditions |
Cool, dry, well-ventilated area |
| Temperature |
Room temperature (15-30°C) |
| Container |
Tightly closed, original packaging |
| Materials to Avoid |
Strong oxidizers, acids, bases, heat |
| Shelf Life |
24 months (under recommended conditions) |
| Packaging |
25 kg bags, 500 kg big bags |
SECTION 11: TRANSPORT INFORMATION
| Parameter |
Information |
| UN Number |
Not regulated (not classified as dangerous goods) |
| Hazard Class |
Not applicable |
| Packing Group |
Not applicable |
| Proper Shipping Name |
N,N'-Methylenebisacrylamide |
| ADR/RID |
Not regulated |
| IMDG Code |
Not regulated |
| IATA (Air) |
Not regulated |
| Marine Pollutant |
No |
SECTION 12: FIRE-FIGHTING MEASURES
| Parameter |
Information |
| Fire Hazard |
Combustible solid |
| Extinguishing Media |
Water fog, CO₂, dry chemical, foam |
| Special Hazards |
When heated, releases nitrogen oxides (NOₓ) and CO₂ |
| Decomposition Products |
Nitrogen oxides, CO, CO₂ |
| Protective Equipment |
Self-contained breathing apparatus (SCBA), full protective clothing |
SECTION 13: ACCIDENTAL RELEASE AND CLEANUP
| Parameter |
Information |
| Personal Protection |
Gloves, safety goggles, dust mask |
| Ventilation |
Increase ventilation |
| Cleanup Methods |
Sweep while preventing dust formation; collect in sealed containers |
| Environmental Precautions |
Prevent entry into drains |
| Waste Disposal |
According to local regulations |
SECTION 14: FIRST AID MEASURES
| Exposure Route |
Action |
| Inhalation |
Move to fresh air. Seek medical attention if symptoms persist. |
| Skin Contact |
Remove contaminated clothing. Wash with soap and plenty of water. |
| Eye Contact |
Rinse with plenty of water for at least 15 minutes. Seek medical attention if irritation persists. |
| Ingestion |
Do NOT induce vomiting. Drink plenty of water. Seek medical attention. |
SECTION 15: REGULATORY INFORMATION
| Region / Authority |
Status |
| EU REACH |
Registered |
| Turkey KKDİK |
Registration required |
| USA TSCA |
Listed |
| EINECS |
203-750-9 |
| WGK (Germany) |
2 (hazardous to water) |
| RoHS |
Compliant |
| California Prop 65 |
Not listed |
SECTION 16: SUMMARY AND CRITICAL WARNINGS
Product Summary
| Parameter |
Value |
| Product |
N,N'-Methylenebisacrylamide (MBA) |
| CAS |
110-26-9 |
| Appearance |
White crystalline particles |
| Molecular Weight |
154.17 g/mol |
| Purity |
≥ 99.0% |
| Key Industries |
Biochemistry, polymer chemistry, water treatment |
CRITICAL WARNINGS
-
Crosslinking Monomer: MBA is an effective crosslinking agent in polymer systems. It is widely used in the formation of polyacrylamide gels and other polymer networks.
-
Gel Electrophoresis: MBA's most important application is as a crosslinker for acrylamide in SDS-PAGE, DNA electrophoresis, and other gel electrophoresis techniques.
-
High Purity: ≥ 99.0% purity makes it suitable for molecular biology and biochemistry applications.
-
Water Soluble: Soluble in water (~30 g/L), ideal for aqueous polymerization systems.
-
Polymerization Risk: Readily polymerizes; contains inhibitor to prevent premature polymerization. Keep away from heat and light.
-
Mutagenicity Suspect: Suspected of causing genetic defects (H341). Handle with caution and use appropriate PPE.
-
Toxic: Harmful if swallowed (H302). Causes skin and eye irritation (H315, H319).
-
Storage: Store in cool, dry, well-ventilated areas. Keep away from strong oxidizers, acids, and bases.
-
Biochemical Grade: Special grades for molecular biology and electrophoresis applications are available (≥ 99.5% purity).
-
Regulatory Status: Registered under REACH. Not classified as dangerous goods but requires careful handling due to mutagenicity suspicion.
IMPORTANT DISCLAIMER: This Technical Data Sheet (TDS) is for informational purposes only and is based on available technical data. The user is solely responsible for determining the product's suitability for their specific application and for complying with all applicable local, national, and international regulations. MBA is suspected of causing genetic defects and readily polymerizes! For complete safety, storage, handling, transportation, disposal, and regulatory compliance information, always refer to the official Safety Data Sheet (SDS/MSDS) provided by the manufacturer/supplier. Users are responsible for testing the product in their own processes and applications. All local, national, and international regulations must be followed when working with MBA.
