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Rennet Casein, Enzyme Coagulated Casein, Rennet Curd Casein, Milk Casein, Bovine Casein, Casein Phosphoprotein, 9000-71-9

Rennet Casein, Enzyme Coagulated Casein, Rennet Curd Casein, Milk Casein, Bovine Casein, Casein Phosphoprotein, 9000-71-9

RENNET CASEIN (MILK CASEIN)
PRODUCT IDENTIFICATION INFORMATION

Parameter Information
Product Name Rennet Casein
Chemical Name Milk Casein (Enzyme Coagulated)
Synonyms (Turkish) Rennet Kazein, Süt Kazeini, Peynir Mayası Kazeini, Enzim Kazein
Synonyms (English) Rennet Casein, Enzyme Coagulated Casein, Rennet Curd Casein, Milk Casein, Bovine Casein, Casein Phosphoprotein
CAS Number 9000-71-9
EC Number (EINECS) 232-555-1
Appearance Yellowish-white to cream-colored, free-flowing granules or powder
Odor Characteristic, mild milky odor

CHEMICAL STRUCTURE

Rennet Casein

Casein Micelle Structure

αs1-CN + αs2-CN + β-CN (CSN2) + κ-CN (Calcium Phosphate Bridges)

Structure: Purified milk protein obtained by coagulating high-quality pasteurized skim milk with rennet enzyme (chymosin); The enzyme cleaves κ-casein, causing casein micelles to aggregate and form a curd; Major protein fractions: αs1-casein, αs2-casein, β-casein, κ-casein; Contains all essential amino acids; High molecular weight phosphoprotein.

SECTION 1: CHEMICAL IDENTITY AND DESCRIPTION

Parameter Information
Product Name Rennet Casein
Chemical Name Milk Casein (Enzyme Coagulated)
Synonyms (Turkish) Rennet Kazein, Süt Kazeini, Peynir Mayası Kazeini, Enzim Kazein
Synonyms (English) Rennet Casein, Enzyme Coagulated Casein, Rennet Curd Casein, Milk Casein, Bovine Casein, Casein Phosphoprotein
CAS Number 9000-71-9
EC Number (EINECS) 232-555-1
Chemical Class Milk protein; Phosphoprotein; Calcium-binding protein
Source Bovine milk (casein protein)
Physical State (20°C) Solid (granules or free-flowing powder)
Odor Characteristic, mild milky
Color Yellowish-white to cream
Taste Mild, slightly milky
Protein Origin Bovine milk (αs1, αs2, β, κ-casein)

SECTION 2: PHYSICAL AND CHEMICAL PROPERTIES

Property Value Test Method / Note
Appearance Yellowish-white to cream-colored, free-flowing granules or powder Visual
Odor Characteristic, mild milky Olfactometric
Protein Content (N×6.38, dry basis) ≥ 88.5% Kjeldahl
Moisture ≤ 12.0% Gravimetric (105°C)
Fat ≤ 1.5% Soxhlet
Ash ≤ 8.5% Gravimetric (550°C)
Lactose ≤ 1.0% HPLC / Enzymatic
pH (10% Solution) 6.8 – 7.5 Potentiometric
Solubility in Water Insoluble (dispersible) General Method
Solubility in Alkaline Soluble (as caseinate) General Method
Solubility in Acid Insoluble (precipitates at pH 4.6) General Method
Isoelectric Point pH ~4.6
Gelation (with rennet) Forms coagulum (curd)
Calcium Content ~2.5 – 3.0%
Phosphorus Content ~0.8 – 1.0%
Bulk Density 0.5 – 0.7 g/cm³
Particle Size Granular (200-500 μm) or powder Sieve / Laser Diffraction
Heavy Metals (as Pb) ≤ 10 ppm AAS / ICP
Lead (Pb) ≤ 1 ppm AAS / ICP
Arsenic (As) ≤ 1 ppm AAS / ICP
Salmonella Absent Microbiological
E. coli Absent Microbiological
Total Plate Count ≤ 10,000 CFU/g Microbiological
Yeast & Mold ≤ 100 CFU/g Microbiological

SECTION 3: PROTEIN FRACTION COMPOSITION

Protein Fraction Content (%) Function
αs1-Casein ~38 – 40 Major protein; Calcium-binding; Emulsification
αs2-Casein ~10 – 12 Calcium-binding; Surface activity
β-Casein ~35 – 40 Surface-active; Emulsification; Water binding
κ-Casein ~10 – 12 Stabilizes micelles; Rennet substrate
Other Proteins ~2 – 5 Minor components; Enzymes

SECTION 4: PRODUCTION PROCESS

Step Process Description
1 Milk Skimming Fresh, high-quality milk is skimmed to separate cream
2 Pasteurization Skim milk is pasteurized (72°C for 15 seconds)
3 Coagulation Rennet enzyme (chymosin) is added at 30-35°C; κ-casein is cleaved; Casein micelles aggregate to form a curd
4 Curd Cutting The curd is cut to release whey
5 Whey Separation Whey is drained; Curd is washed with water
6 Dewatering Curd is dewatered (pressed or centrifuged)
7 Drying Curd is dried (typically at 50-70°C)
8 Milling Dried casein is milled to granules or powder
9 Packaging Packaged in moisture-proof containers

SECTION 5: FUNCTIONAL PROPERTIES AND MECHANISM OF ACTION

Property Description
Water Binding Binds water effectively; Forms a gel structure when hydrated; Prevents syneresis; Improves texture and juiciness
Fat Binding Binds fat in emulsions; Stabilizes oil-water systems; Essential for cheese and meat products
Emulsification Stabilizes emulsions; Forms stable fat-protein complexes; Used in processed cheese, sausages, and creamers
Gel Formation Forms coagulum with rennet; Creates a three-dimensional protein network; Essential for cheese texture
Film Former Forms edible films and coatings; Used in food packaging and encapsulation
Slow Digestion Forms a gel in the stomach (with acid); Amino acids are released slowly over 6-8 hours; Anti-catabolic effect (muscle preservation)
BCAA Rich High content of leucine, isoleucine, valine; Supports muscle recovery and growth
Phosphopeptides Contains natural bioactive peptides; Supports calcium and mineral absorption
Heat Stability Stable at high temperatures; Suitable for cooked and baked products

SECTION 6: AMINO ACID PROFILE (g/100g protein)

Essential Amino Acid Amount Essential Amino Acid Amount
Histidine ~2.8 Lysine ~7.5
Isoleucine ~5.0 Methionine ~2.5
Leucine ~9.0 Phenylalanine ~4.5
Threonine ~4.5 Valine ~6.5
Tryptophan ~1.2

BCAA Content: High (Leucine ~9%, Isoleucine ~5%, Valine ~6.5%)

SECTION 7: MAIN APPLICATION AREAS

Industry / Application Function Typical Use Rate (%)
Processed Cheese Water/fat binding, emulsification, stretchability 3 – 8%
Fermented Dairy (Yogurt, Ayran) Viscosity enhancer, syneresis prevention 1 – 3%
Meat Products (Sausages, Salami) Emulsion stability, water retention, cooking loss reduction 2 – 5%
Sports Nutrition Slow-digesting protein, BCAA source, anti-catabolic 10 – 100% (protein blend)
Cosmetics Film former, skin elasticity, moisturizing 0.5 – 2.0%
Industrial (Paper, Coatings, Adhesives) Film forming, binding, adhesion 1 – 5%
Clinical Nutrition Medical nutrition, tube feeding 5 – 20%
Confectionery Binder, texture 1 – 3%
Bakery Water retention, texture 1 – 3%

SECTION 8: SAMPLE FORMULATIONS

8.1 Processed Cheese (Melting Cheese, Mozzarella Style)

Component Low Cost (Analog) Standard Quality Function
Fresh Curd / Aged Cheese Base 40 – 50% 60 – 70% Base
Rennet Casein 6 – 8% 3 – 5% Structure, stretch
Emulsifying Salt (E331, E452) 2.5 – 3.0% 2.0 – 2.5% Emulsification
Vegetable Oil / Butter 15 – 20% 10 – 15% Fat
Water / Whey Powder Balance Balance Moisture
Salt (NaCl) 1.0 – 1.5% 0.8 – 1.2% Flavor

Instructions: Mix Rennet Casein with emulsifying salts; Heat cheese base in cutter to 80-85°C; Add dry mixture slowly; Mix at high speed until smooth (4-8 minutes).

8.2 Sausage / Salami Formulation (100 kg Meat Base)

Component Amount Function
Beef/Meat (with fat) 100 kg Base
Rennet Casein 1.5 – 2.5 kg Binder, water retention
Salt 2.0 – 2.5 kg Curing, flavor
Garlic 0.5 – 1.0 kg Flavor
Spice Blend As required Flavor
Ice Water 5 – 10 kg Hydration

Instructions: Dry mix Rennet Casein with salt and spices before adding to meat; Add ice water for hydration; Mix well.

8.3 Night-Time Protein Blend (30g Serving)

Component Amount (g) Function
Rennet Casein 20 – 25 g Slow-release protein
Cocoa Powder (Alkalized) 3 – 5 g Flavor, color
Sunflower Lecithin (Powder) 0.5 g Solubility, flowability
Sweetener / Flavor As required Taste

Instructions: Mix all ingredients; Consume 30-45 minutes before bed with 250-300 ml water or milk.

SECTION 9: USAGE INSTRUCTIONS

General Dosing Guide:

Application Dosage (%) Form
Processed Cheese 3 – 8 Granules/Powder
Fermented Dairy 1 – 3 Powder
Meat Products 2 – 5 Powder
Sports Nutrition 10 – 100 Powder
Cosmetics 0.5 – 2.0 Powder
Industrial 1 – 5 Powder

Instructions:

  1. Hydration: Rennet Casein absorbs water slowly; Allow sufficient hydration time (5-10 minutes).

  2. Temperature: For processed cheese, heat to 80-85°C for proper hydration and emulsification.

  3. pH: Optimum functionality at pH 6.8-7.5; Insoluble below pH 4.6 (isoelectric point).

  4. Mixing: For meat products, dry mix with salt and spices before adding to meat; Prevents clumping.

  5. Sports Nutrition: Does not dissolve (forms suspension); Shake well before consumption.

SECTION 10: QUALITY SPECIFICATIONS

Parameter Food Grade Standard
Appearance Yellowish-white to cream granules/powder Visual
Protein Content (N×6.38, dry basis) ≥ 88.5% Kjeldahl
Moisture ≤ 12.0% Gravimetric
Fat ≤ 1.5% Soxhlet
Ash ≤ 8.5% Gravimetric
Lactose ≤ 1.0% HPLC
pH (10% Solution) 6.8 – 7.5 pH Meter
Heavy Metals (as Pb) ≤ 10 ppm AAS / ICP
Lead (Pb) ≤ 1 ppm AAS / ICP
Arsenic (As) ≤ 1 ppm AAS / ICP
Salmonella Absent Microbiological
E. coli Absent Microbiological
Total Plate Count ≤ 10,000 CFU/g Microbiological
Yeast & Mold ≤ 100 CFU/g Microbiological

SECTION 11: TOXICOLOGICAL PROFILE

Parameter Value
Acute Oral Toxicity (LD50, Rat) > 10,000 mg/kg (practically non-toxic)
Acute Dermal Toxicity Non-irritating
Skin Irritation Non-irritating
Eye Irritation May cause mechanical irritation (powder)
Allergenicity Contains milk protein – contraindicated for milk allergy sufferers
Chronic Toxicity Non-toxic; FDA GRAS status
Carcinogenicity Not classified as carcinogenic
Mutagenicity Not mutagenic
Target Organs None (non-toxic)
Special Toxicity Note Non-toxic; FDA GRAS; Contains milk allergen – must be labeled for milk allergy; Not suitable for lactose-free diets (contains trace lactose); High-quality complete protein; Natural calcium and phosphorus source

SECTION 12: GHS CLASSIFICATION AND LABELING

GHS Classification (in accordance with CLP and UN GHS):

Hazard Class Category H-Statement
Not classified as hazardous

SIGNAL WORD: NONE (not classified as hazardous)

Hazard Pictograms: NONE

Hazard Statements (H-Codes): Not classified as hazardous under GHS regulations.

NFPA 704 Hazard Classification:

Health Flammability Reactivity Special
1 1 0 -
(Slight health hazard – dust) (Combustible – dust may form explosive mixture) (Stable) (None)

SECTION 13: PRECAUTIONARY STATEMENTS (P-CODES)

Code Statement
P261 Avoid breathing dust/fume/gas/mist/vapors/spray
P264 Wash hands thoroughly after handling
P271 Use only outdoors or in a well-ventilated area
P280 Wear protective gloves/protective clothing/eye protection/face protection
P302+P352 IF ON SKIN: Wash with plenty of soap and water
P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing
P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and continue rinsing
P312 Call a POISON CENTER/doctor if you feel unwell
P332+P313 If skin irritation occurs: Get medical advice/attention
P337+P313 If eye irritation persists: Get medical advice/attention
P362+P364 Take off contaminated clothing and wash it before reuse
P403+P233 Store in a well-ventilated place. Keep container tightly closed
P501 Dispose of contents/container in accordance with local/regional/national/international regulations

SECTION 14: FIRST AID MEASURES

Exposure Route Action to Take
Inhalation Remove to fresh air immediately. If respiratory irritation develops (dust inhalation), seek medical attention.
Skin Contact Wash with plenty of soap and water. If irritation persists, get medical attention.
Eye Contact Rinse immediately with plenty of water for at least 15 minutes. Keep eyelids open. Remove contact lenses if present. If irritation persists, seek medical attention.
Ingestion Rinse mouth. Drink plenty of water. Generally non-toxic. If large quantities are ingested and symptoms occur, seek medical attention.

SECTION 15: FIREFIGHTING MEASURES

Parameter Information
Fire Hazard Combustible organic material (high protein content). Dust cloud may form explosive mixture with air (if finely dispersed).
Hazards Arising from Fire Thermal decomposition produces carbon monoxide, carbon dioxide, nitrogen oxides (NOx).
Suitable Extinguishing Media Water spray, CO₂, dry chemical powder, foam.
Unsuitable Extinguishing Media Water jet (may spread dust)
Special Protective Equipment Self-contained breathing apparatus (SCBA), full protective clothing (for surrounding fire).
Special Precautions Prevent dust from entering sewers and waterways. Avoid dust cloud formation.

SECTION 16: STORAGE AND SHELF LIFE

Parameter Information
Storage Conditions Store in a cool, dry, well-ventilated area; PROTECT FROM MOISTURE and high humidity; Keep away from direct sunlight and high temperatures; Storage temperature 10-25°C
Container Requirements Tightly closed, moisture-proof containers (food-grade multi-layer kraft bags with PE inner liner, fiber drums, HDPE containers)
Materials to Protect From Moisture, high humidity, light, strong oxidizing agents, direct heat sources
Shelf Life 24 months (in unopened original packaging, under proper storage conditions)
Stability Note Hygroscopic – absorbs moisture; Protein may degrade at high temperatures or with prolonged storage; Keep container tightly closed after opening; Store in cool, dry place; Avoid odor absorption
Special Storage Requirements Store in a dry, well-ventilated warehouse; Avoid exposure to rain, sun, and moisture; Opened containers must be resealed immediately

SECTION 17: PACKAGING OPTIONS

Packaging Type Quantity Material
Multi-layer Paper Bag 20 kg, 25 kg PE-lined kraft paper bag (moisture-proof)
Plastic Bag 5 kg, 10 kg, 25 kg PE bag (inner-lined, sealed, moisture-proof)
Fiber Drum 20 kg, 25 kg PE-lined fiberboard drum
Plastic Drum 25 kg, 50 kg HDPE / Polypropylene (moisture-proof)
FIBC / Big Bag 500 kg, 1000 kg PE-lined, moisture-resistant PP woven bag

SECTION 18: TRANSPORT INFORMATION

Parameter Information
UN Number Not regulated (non-hazardous)
Hazard Class Not applicable
Packing Group Not applicable
ADR/RID Not regulated
IMDG Code Not regulated (non-hazardous)
IATA (Air) Not regulated (non-hazardous)
Marine Pollutant No
Transport Temperature Ambient temperature; Protect from moisture.

SECTION 19: REGULATORY STATUS

Region / Authority Status
European Union Food ingredient approved; Milk protein; Not classified as hazardous
USA (TSCA) Listed (TSCA Inventory)
USA (FDA) GRAS (Generally Recognized as Safe); Milk protein; Food ingredient
Turkey Food ingredient; Subject to KKDIK regulation; Milk product regulations
IARC Not classified (non-toxic, non-carcinogenic)
NTP Not listed
OSHA Not regulated as toxic or carcinogenic substance
California (Prop 65) Not listed
Halal Yes (with Halal certification; requires microbial/vegetable rennet)
Kosher Yes (with Kosher certification; requires microbial/vegetable rennet)
Allergen Labeling Must be labeled as containing milk (milk allergy warning)
GMO Non-GMO (certified non-GMO raw materials)
Irradiation Not irradiated

SECTION 20: OTHER NAMES AND SYNONYMS

Category Names
Turkish Names Rennet Kazein, Süt Kazeini, Peynir Mayası Kazeini, Enzim Kazein
English Names Rennet Casein, Enzyme Coagulated Casein, Rennet Curd Casein, Milk Casein, Bovine Casein, Casein Phosphoprotein
Biological Names Bovine Casein Phosphoprotein, αs1-CN, αs2-CN, β-CN, κ-CN
CAS Number 9000-71-9
EC Number 232-555-1
Commercial Names Milk Protein, Bovine Milk Protein, Curd Casein, Rennet Curd
Synonyms Süt Kazeini, Casein Phosphoprotein, Casein Rennet

SECTION 21: SUMMARY TABLE

Parameter Value
Product Rennet Casein
CAS Number 9000-71-9
Protein Content ≥ 88.5%
Appearance Yellowish-white to cream granules/powder
pH (10% Solution) 6.8 – 7.5
Isoelectric Point pH ~4.6
Primary Function Water/fat binding, gelation, slow-release protein
Main Application Areas Processed cheese, meat products, sports nutrition, fermented dairy, cosmetics, industrial
Shelf Life 24 months
GHS Signal Word NONE (not classified as hazardous)
UN Number Not regulated
Special Properties Milk protein; Enzyme-coagulated; Complete protein; Slow digestion (6-8 hours); BCAA-rich; Allergen (milk); Non-GMO; Not irradiated

SECTION 22: CRITICAL WARNINGS AND BEST PRACTICES

CRITICAL WARNINGS:

  • Not classified as hazardous under GHS. Non-toxic, non-carcinogenic. FDA GRAS status.

  • Contains milk allergen – contraindicated for milk allergy sufferers. Must be labeled accordingly.

  • Hygroscopic – absorbs moisture from air. Store in tightly closed, moisture-proof containers.

  • Dust may cause mechanical irritation to eyes, skin, and respiratory system. Use appropriate dust control measures.

  • Combustible (organic material, high protein content). Keep away from open flames and high heat. Dust cloud may form explosive mixture.

  • Slow hydration – requires sufficient hydration time (5-10 minutes) and temperature (80-85°C for cheese).

  • Insoluble in water – forms suspension, not solution (unlike sodium caseinate).

  • Slow digestion – forms a gel in the stomach; Amino acids released over 6-8 hours; Anti-catabolic effect.

  • Not suitable for lactose-free diets – contains trace lactose (<1%).

  • Not suitable for milk-allergic individuals – contains casein protein.

  • Rennet source – enzyme-coagulated (not acid-coagulated); Different functionality from acid casein.

BEST PRACTICE RECOMMENDATIONS:

Processed Cheese: Use as water/fat binder and texturizer; Dosage 3-8%; Mix with emulsifying salts before adding; Heat to 80-85°C for complete hydration; Essential for stretch and sliceability; Prevents oiling-off.

Meat Products: Use as binder and water retention agent; Dosage 2-5%; Dry mix with salt and spices before adding to meat; Reduces cooking loss; Improves sliceability.

Fermented Dairy (Yogurt, Ayran): Use as viscosity enhancer and syneresis prevention; Dosage 1-3%; Adds body and texture; Prevents whey separation.

Sports Nutrition: Use as slow-release protein; Dosage 10-30 g per serving; Best consumed 30-45 minutes before bed; Mix with water or milk; Forms a gel in stomach; Provides sustained amino acid release (6-8 hours).

Cosmetics: Use as film former and moisturizer; Dosage 0.5-2.0%; pH 7.0 optimal; Provides skin elasticity and repair.

Industrial (Paper, Coatings): Use as binder and film former; Dosage 1-5%; Water-based film formation; Adhesion properties.

Handling: Use in well-ventilated areas; Avoid dust formation; Use dust masks (N95/FFP2) when handling powder; Use gloves and safety goggles.

Personal Protective Equipment: Dust mask (N95/FFP2 or higher), safety goggles, protective gloves (nitrile, neoprene). Not required for solution handling.

Hygiene: Wash hands thoroughly after handling; Do not eat, drink, or smoke while using.

Storage: Store in tightly closed, moisture-proof containers; Store in a cool, dry, well-ventilated area (10-25°C); Protect from moisture, light, and heat; Opened containers must be resealed immediately; Shelf life: 24 months under proper conditions.

Spill Management: Sweep or vacuum (HEPA) and place in suitable sealed container; Avoid dust formation; Do not allow entry into drains or waterways.

Disposal: Dispose of in accordance with local, regional, and national regulations; Non-hazardous waste; Biodegradable; Can be disposed of in compliance with regulations.

RENNET CASEIN vs ACID CASEIN COMPARISON:

Property Rennet Casein Acid Casein
Coagulation Method Rennet enzyme (chymosin) Acid (HCl or H₂SO₄)
pH 6.8 – 7.5 4.5 – 5.0
Calcium Content High (~2.5-3.0%) Low (~0.1-0.2%)
Water Solubility Insoluble (dispersible) Insoluble
Gel Formation Good (curd formation) Poor
Cheese Stretch Excellent Poor
Primary Use Processed cheese, sports nutrition Caseinates, technical applications

RENNET CASEIN vs SODIUM CASEINATE COMPARISON:

Property Rennet Casein Sodium Caseinate
Solubility in Water Insoluble (suspension) Fully soluble
Gel Formation Yes (with acid) No
Digestion Rate Slow (6-8 hours) Fast
Primary Use Cheese, sports nutrition (night) Creamers, sauces, sports (post-workout)

QUICK REFERENCE TABLE:

Issue Solution / Explanation
Cheese too hard, no stretch Rennet Casein ratio too high, fat too low; Increase emulsifying salt by 0.5% or add 2-3% water
Bitter taste in cheese Rennet Casein may be old or stored at high temperature (proteolysis); Check storage conditions
Sports drink too thick (pudding-like) Normal for Rennet Casein; Increase water from 300 ml to 400 ml for thinner consistency
Can I use for pastrami injection? No – particle size too large; Use Sodium Caseinate for brine injection

LEGAL DISCLAIMER:
The information provided in this document is based on our current knowledge and experience and is presented in good faith. However, as all conditions of use are beyond our control, it does not constitute a binding specification or warranty. This Technical Data Sheet is for informational purposes only. Users are responsible for testing the suitability for their own applications. For complete safety, storage, use, handling, waste, and regulatory compliance information, please refer to the official Safety Data Sheet (SDS/MSDS) provided by the manufacturer/supplier. This product contains milk protein and must be labeled accordingly for milk allergy. The usage rates provided are recommendations only and may vary depending on formulation, equipment, and local regulations.

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